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Biozellen®3D类器官培养基质胶套装

一、应用•3D类器官培养。•3D细胞球体培养试验•细胞侵袭•细胞迁移•小鼠皮下成瘤•适合用于3D细胞药物筛检平台•细胞生长和分化•代谢/毒理学研究•体外和体内血管生成分析二、适用细胞种类• 原代细胞 • 干细胞


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成功转染含有Mincle过表达质粒pcDNA3.1到骨髓源巨噬细胞(BMDM)发表文章已见刊

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Zeta Life转染试剂成功转染含有Mincle过表达质粒pcDNA3.1到骨髓源巨噬细胞(BMDM)西南医科大学,中西医结合研究中心科研工作者发表文章用Zeta Life Advanced DNA RNA,AD600150转染试剂成功转染含有Mincle过表达质粒pcDNA3.1到骨髓源巨噬细胞(BMDM),实验数据见下:. A&P inhibits the Mincle/Syk/NF-κB signaling pathway and M1 macrophage in vitro. The protein levels of each indicator in Western blot assay(A); The mRNA expression level of Mincle(B); The Flow results of Mincle, iNOS and CD206 in macrophages(C). *P < 0.05, **P < 0.01, ***P < 0.001 vs. LPS group; #P < 0.01 vs.Control group.用Zeta Life Advanced DNA RNA,AD600150转染试剂转染含有Mincle过表达质粒pcDNA3.1到骨髓源巨噬细胞(BMDM)的部分文章。

浙江大学高效率转染质粒DNA、siRNA到免疫T(Vδ1 T)细胞,发表文章到nature-sigtrans

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一、浙江大学附属第二医院乳腺外科;浙江大学附属第二医院浙江省疗法肿瘤微环境与免疫重点实验室;浙江省人民医院,浙江省人民医院,杭州医学院附属人民医院,浙江省个体化医学肿瘤分子诊断与诊断重点实验室;绍兴大学附属医院甲状腺乳腺外科联合发表标题为Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells(乳腺癌衍生的外泌体传递lncRNA SNHG16诱导CD73 +γδ1 Treg细胞)的文章到nature.com/sigtrans,文章已被接受;二、本研究使用中使用Invigentech(英克)公司INVI DNA RNA 转染试剂转染质粒DNA、siRNA到Vδ1 T 免疫T细胞里面;三、本文中转染质粒DNA、siRNA,转染细胞数量信息如下:A. 将全长2435 bp的序列克隆到pCR3.1载体中构建SNHG16过表达载体;B. siRNA序列:SNHG16-homo-349,5′GCCUCUGCUGCUAAUUGUUTT-3'; SNHG16-homo-867, 5′-CCAAGGAGGGACUGUUUAATT-3′和SNHG16-homo-2004,5′-CCCAGUGUUGACUCACCAATT-3;C. 转染细胞用量:6孔板里面1 × 106 cells/well;四、发表文章部分内容如下:Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cellsPlasmid construction and siRNA silencing A full-length 2435 bp sequence was cloned into the pCR3.1 vector toconstruct the SNHG16 overexpression vector. The sequences of SNHG16-specifific siRNAs were as follows: SNHG16-homo-349, 5′GCCUCUGCUGCUAAUUGUUTT-3′; SNHG16-homo-867, 5′-CCAAGGAGGGACUGUUUAATT-3′ and SNHG16-homo-2004, 5′-CCCAGUGUUGACUCACCAATT-3′. The miR-16–5p mimics, inhibitor and negativecontrols were purchased from GenePharma (SupplementaryTable S3).To manipulate the expression of miR-16–5p in Vδ1 T cells, Vδ1T cells were sorted from peripheral blood and seeded into six-wellplates at 1 × 106 cells/well with 1 ml of medium supplementedwith 10% FBS, 10 μg/ml CD3, 10 μg/ml CD28 and 40 U/mL IL-2.Then, transfection reagent (INVI DNA RNA Transfection Reagent,Invigentech, USA) and miR-16–5p mimics/NC/inhibitor/inhibitor NC (20 μm) were incubated with the cells for 48 h, after which the cells were collected for further experiments.but not Vδ2+ T cells were revealed to comprise the majority of TILs in all three BC molecular subtypes when gated on CD3 (p < 0.01, Fig. 1b, c). In addition, immunoflfluorescence of frozen sections further confifirmed the dominant distribution of Vδ1+T cells in BC (Fig. 1d). To further identify the function of breast cancer-infifiltrating γδ1T cells, BC cell lines (MCF-7, T-47D, MDA-MB-231 and MDA-MB-468) were co-cultured with freshly isolated Vδ1+ T cells frombreast tumour tissue (E:T ratios: 1:1, 5:1 and 10:1). Upon plotting the cell growth curve, the data show that γδ1 T cells did not exertinvigentech(英克)INVI DNA RNA转染试剂信息如下产品名称货号规格报价INVI DNA RNA 转染试剂IV12160250.25 ml询价INVI DNA RNA 转染试剂IV12160500.50 ml询价INVI DNA RNA 转染试剂IV12160750.75 ml询价INVI DNA RNA 转染试剂IV12161001.00 ml询价INVI DNA RNA 转染试剂IV12161501.50 ml询价INVI DNA RNA 转染试剂IV12163003.00 ml询价www.ivigt.com

转染原代破骨细胞前体细胞,在已发表期刊文章中使用invigentech转染试剂的转染经验分享

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西部战区总医院药学部,在2021年发表文章到Cell Communication and Signaling的学术期刊中科研者使用美国invigentech转染试剂,高效率转染原代破骨细胞前体细胞(Osteoclast precursor cells),具体转染原代破骨细胞前体细胞方法经验如下:(注意:本文转染实验中使用的细胞数量、试剂用量和操作方法不适用于传统lipo3000转染试剂或类似产品)。Cell cultureSorted primary CD115(+) precursors were cultured in α-minimal essential medium (MEM) containing 10% FBS,1% penicillin-streptomycin solution and 50 ng ml− 1 colony stimulating factor (M-CSF, Abcam). After 3 days,the medium was replaced with fresh medium. LA was added at a concentration of 100 μg/ml to stimulate the precursors. To inhibit the expression of Cadherin-11, the primary osteoclast precursors were transfected with Cadherin-11 siRNA using INVI DNA RNA Transfection reagent (Invigentech) for 24 h. The sequence of Cadherin-11 siRNA was CCAATGGACCAAGATTTAT. In some experiments, primary cells were treated with BYL719 (2.5 μM), GSK690693 (25 nM), 666–15 (50 nM), rapamycin (20 μM), or AMG-487 (30 μM).Invigentech.英克: 简介美国 Invigentech(英克)公司是开发用于细胞培养的胎牛血清、细胞活力检测、细胞筛选、细胞支原体清除、细胞转染和组织再生的新型血清替代品全球***之一;提供inviCELL™Platelet lysate无动物血清产品用于支持广泛的细胞扩增和生产,包括培养间充质干细胞和多种免疫细胞系等,为制药公司或者生物技术公司提供无血清细胞培养规模生产服务;INVI DNA  RNA Transfection Reagent为原代细胞、悬浮细胞、小动物活体转染提供高品质转染试剂;此外我们引进的新设施可生产高纯度注射用水和生物处理解决方案,用于满足任何细胞、科研和企业客户需求,保证每个指定批次胎牛血清、细胞活力检测、细胞筛选细胞,转染等产品质量的稳定性,以加速安全有效的基于细胞和组织的治疗研究、开发和商业化。www.ivigt.com

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新产品活动,回报广大客户的信任选择!
Zeta life活动送-86超低温冰箱
选择下面Zeta Life产品数量满15个可参加本活动一、胎牛血清 货号Z7180FBS-500 数量5瓶二、DNA、RNA转染试剂 货号AD600150 数量5盒三、ECL液-WB化学发光液 货号:310209-500 数量5套(本产品选择数量*多5套)
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